Maintaining cultures of ectomycorrhizal and plant pathogenic fungi in sterile water cold storage

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Abstract
Mycelial cultures of 64 isolates of 14 spp. of ectomycorrhizal fungi [Boletinus meruloides, Cenococcum graniforme, Laccaria laccata, Lepiota nuda, Pisolithus tinctorius, Poria terrestris, Rhizopogon roseolus, Scleroderma bovista, Suillus albidipes, S. grevillei, S. luteus, Theleophora terrestris] and 27 isolates of 15 spp. of plant pathogenic fungi [Armillaria mellea, Fusarium oxysporum f. pini, Polyporus tomeatosus var. circinatus, Poria weirii, Phytophthora cactorium, P. cinnamomi, P. citricola, P. diechsleri, P. heveae, P. parasitica, Phythium debrayanum, P. dissotocum, P. irregulare, P. spinosum, P. vexans] were grown on agar medium in Petri dishes. Mycelial discs, 8 mm in diameter, were removed from the cultures and stored in sterile distilled water in test tubes at 5.degree. C. Sixty-four, 61 and 41 isolates of the symbiotic fungi were viable after 1, 2 and 3 yr storage, respectively. Only 19, 10 and 8 isolates of the pathogenic fungi were viable after 1, 2, and 3 yr storage, respectively. Time in pure culture before water storage did not affect viability of any fungal species following water storage. After 3 yr storage, 4 fungi (3 symbionts and 1 pathogen) retained their original growth rates and root-infecting abilities on pine seedlings. The same 4 isolates however, maintained on agar slants at 5.degree. C and subcultured every 4-6 mo., grew slower and did not infect as many feeder roots of pine as the water-stored isolates.

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