Effects of Different Levels of Glucose upon the Development of Granulated Beta Cells in Cultures of Pancreatic Primordia from Normal Rat Embryos
- 1 December 1967
- journal article
- research article
- Published by American Diabetes Association in Diabetes
- Vol. 16 (12) , 839-845
- https://doi.org/10.2337/diab.16.12.839
Abstract
Pancreatic primordia from normal rat embryos of 14.5 to 15.5 days postcoitum were grown in organ culture on liquid media with 173, 350, 528 and 995 mg of glucose/100 ml. The periods of culture were as follows 8 days (14.5 days embryos) and 6 days (15.5 day embryos). In cultures grown on the standard medium with 173 mg of glucose/100 ml, the pancreatic islets had many granulated beta cells. In cultures grown on high-glucose media with 350, 526 and 995 mg of glucose /100 ml the islets had fewer granulated beta cells. These observations are interpreted to mean that high-glucose levels in the media inhibit the production of granulated beta cells in the islets of the cultures.This publication has 1 reference indexed in Scilit:
- Development of islets and acini from the explanted primordium of the rat embryoThe Anatomical Record, 1965