Localization of 3' ends of 5S and 23S rRNAs in reconstituted subunits of Escherichia coli ribosomes.
- 1 September 1981
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (9) , 5538-5542
- https://doi.org/10.1073/pnas.78.9.5538
Abstract
Periodate-oxidized 3' ends of 5S, 23S, and 16S rRNAs from Escherichia coli were allowed to react with fluorescein thiosemicarbazide, then labeled rRNAs were reconstituted into active ribosomal subunits. The fluorescein moiety on each of the rRNAs when reconstituted into ribosomal subunits was accessible to anti-fluorescein IgG as determined by fluorescence quenching and by sucrose gradient centrifugation. The region at which an antibody molecule bond to the labeled ribosomal subunits was determined by immunoelectron microscopy. The 3' end of the 5S RNA was localized on the central protuberance of the 50S subunit. The corresponding region for the 3' end of the 23S RNA was below the stalk on the noninterfacing surface. The 3' end of the 16S RNA was localized to the upper edge of the large lobe of 30S subunits, as reported previously.Keywords
This publication has 15 references indexed in Scilit:
- Ribosome structure: localization of 3' end of RNA in small subunit by immunoelectronmicroscopy.Proceedings of the National Academy of Sciences, 1979
- Localization of the decoding region on the 30S Escherichia coli ribosomal subunit by affinity immunoelectron microscopy.Proceedings of the National Academy of Sciences, 1979
- Localization of ribosomal proteins L6L12 in the 50 S subunit of Escherichia coli ribosomes by electron microscopyJournal of Molecular Biology, 1976
- Ribosome structure determined by electron microscopy of Escherichia coli small subunits, large subunits and monomeric ribosomesJournal of Molecular Biology, 1976
- Architecture of the Escherichia coli ribosome as determined by immune electron microscopy.Proceedings of the National Academy of Sciences, 1975
- 30S Ribosomal proteins associated with the 3′‐terminus of 16S RNAFEBS Letters, 1975
- Determination of the location of proteins L14, L17, L18, L19, L22 and L23 on the surface of the 50S ribosomal subunit of Escherichia coli by immune electron microscopyMolecular Genetics and Genomics, 1974
- Identification of neighbor relationships among proteins in the 30 S ribosome: Intermolecular cross-linkage of three proteins induced by tetranitromethaneJournal of Molecular Biology, 1973
- Observations on the shape of the 50S ribosomal subunit.Proceedings of the National Academy of Sciences, 1968
- Dependence of the chemical nature of antibodies on the net electrical charge of antigens.Proceedings of the National Academy of Sciences, 1966