Transfer of symbiotically fixed nitrogen from berseem (Trifolium alexandrinumL.) to maize via vesicular—arbuscular mycorrhizal hyphae
Open Access
- 1 November 1992
- journal article
- Published by Wiley in New Phytologist
- Vol. 122 (3) , 447-454
- https://doi.org/10.1111/j.1469-8137.1992.tb00072.x
Abstract
Summary: In order to examine whether hyphae of VA mycorrhizal fungi may aid the transfer of symbiotically fixed nitrogen from a legume to a non‐legume, the roots of berseem plants inoculated with the VAM fungusGlomus intraradicesandRhizobium leguminosarumbiovar.trifolii(strain RCR 5) were separated by a 2 cm root‐free zone from the roots of maize plants. A 40 um pore size nylon net allowed VAM hyphae to pass through the root‐free zone but prevented root penetration. Replacement of this net by a 0.45 μm pore size membrane prevented penetration of VAM hyphae to the root‐free compartment. When the berseem plants were 63 d old, the plant chambers were sealed in plastic bags to allow the roots of the plants to be exposed to al0N2‐enriched atmosphere for 5 d.Berseem plants that were exposed to a15N2‐enriched atmosphere fixed more15N than did berseem plants maintained under air at natural15N abundance. The total15N excess content of berseem was similar whether or not the mycorrhiza had access to the maize roots. Plant mass (dry weight) of maize was not affected by the mycorrhizal fungus, but total nitrogen content tended to be higher in VAM‐colonized maize than in non‐colonized maize. Furthermore, both atom %15N excess and15N excess content of mycorrhizal maize were significantly increased as compared with non‐mycorrhizal maize or with maize controls without berseem. The amounts of15N transferred were small, accounting for less than 4% of the15N in the N2‐fixing plant.In spite of the 2 cm root‐free separating zone, the presence of the mycorrhizal fungus in the non‐legume increased the transfer of symbiotically fixed nitrogen from berseem to maize plants that were physiologically younger. The implications of these results for nitrogen transfer from a legume to an accompanying non‐legume via VAM hyphae are discussed.Keywords
This publication has 45 references indexed in Scilit:
- The stress‐gradient hypothesis does not fit all relationships between plant–plant interactions and abiotic stress: further insights from arid environmentsJournal of Ecology, 2005
- Nutrient transfer between the root zones of soybean and maize plants connected by a common mycorrhizal myceliumPhysiologia Plantarum, 1991
- Nutrient transfer between the root zones of soybean and maize plants connected by a common mycorrhizal myceliumPhysiologia Plantarum, 1991
- A method for measuring hyphal nutrient and water uptake in mycorrhizal plantsCanadian Journal of Botany, 1991
- Hyphae of a vesicular—arbuscular mycorrhizal fungus maintain infectivity in dry soil, except when the soil is disturbedNew Phytologist, 1989
- A new technique for monitoring hyphal growth of vesicular-arbuscular mycorrhizal fungi through soilTransactions of the British Mycological Society, 1987
- A method for measuring the transfer of fixed nitrogen from free-living bacteria to higher plants using 15N2Journal of Microbiological Methods, 1984
- HYPHAL UPTAKE AND TRANSPORT OF NITROGEN FROM TWO 15N‐LABELLED SOURCES BY GLOMUS MOSSEAE, A VESICULAR‐ARBUSCULAR MYCORRHIZAL FUNGUS *New Phytologist, 1983
- Carbon flow, photosynthesis, and N2 fixation in mycorrhizal and nodulated faba beans (Vicia faba L.)Soil Biology and Biochemistry, 1982
- TRANSLOCATION AND TRANSFER OF NUTRIENTS IN VESICULAR‐ARBUSCULAR MYCORRHIZASNew Phytologist, 1981