THE PREPARATION OF SUCROSE-C14WITH HIGH SPECIFIC ACTIVITY AND HIGH TRACER YIELD USING DETACHED SUGAR BEET LEAVES
- 1 February 1959
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 37 (2) , 193-197
- https://doi.org/10.1139/o59-021
Abstract
Radioactive sucrose, in millicurie amounts, was prepared by feeding a detached sugar beet leaf C14O2in air for 15 to 20 minutes followed by 5 to 20 minutes additional photosynthesis in air. The low endogenous sucrose in the leaf coupled with the improved tracer yield effected by the displacement technique gave sucrose with high specific activity. This sucrose-C14, hydrolyzed by 1% invertase, was a good source for radioactive glucose and fructose. Paper chromatographic procedures were used for the isolation and purification of the sugars.Keywords
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