An Investigation of the Antitumor Activity of Micromelum integerrimum (Rutaceae)

Abstract
Extracts of M. integerrimum (Buch.-Ham. ex Coleb.) M. Roem. were fractionated based on in vivo activity in mice in the [mouse] P-388 lymphocytic leukemia cells system. Activity in ethanol extracts was concentrated in the chloroform partition fraction, which was further resolved by chromatography on silica gel. The known coumarins, micromelin and scopoletin, were crystallized from the active fractions and demonstrated to have antitumor activities. Micromelin was converted to the corresponding butenolide (deoxymicromelin) which was inactive in the 9KB [human nasopharyngeal carcinoma cells] assay.

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