The State of Actin in Activated Human Platelets

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Abstract
The state of platelet actin was determined using a fluorescent method of DNase I inhibition. Activation of human platelets resulted in mobilization of DNase-available actin. When platelets were activated with ADP the change in the state of actin was gradual and preceded the secretion. When thrombin was used as an activator, a sharp and rapid decrease in the DNase-available actin was observed which paralleled the secretion. Inhibition of ADP-induced aggregation (and secretion) by EDTA resulted in a decrease in the rate of change on actin. Inhibition of the thrombin-induced aggregation (but not secretion) by EDTA did not affect the change in the state of actin.