Interactions between nitrogen oxide‐containing compounds and peripheral benzodiazepine receptors
- 29 January 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 260 (2) , 169-172
- https://doi.org/10.1016/0014-5793(90)80095-z
Abstract
Nitrogen oxide-containing compounds displaced the peripheral benzodiazepine ligand [3H]Ro5-4864 from guinea pig membrane preparations. Sodium nitroprusside (SNP) was the most potent (IC 50 = 5.61 ± 1.72 × 10−5 M). Moreover, its ability to bind to these receptors showed marked tissue variability (heart > kidney ⪢ cerebral cortex). When tested on rat atrium, SNP by itself had no effect on basal inotropy or the increase in inotropy induced by (−)-S-BAY K 8644. In contrast, Ro5-4864 potentiated the marked increase in inotropy induced by (−)-S-Bay K 8644, and SNP completely abolished the potentiation of inotropy observed with Ro5-4864. Since peripheral benzodiazepine receptors are associated with calcium mobilization in the heart, these findings may indicate that some of the clinical effects of nitric oxide-generating drugs could be mediated by these receptors.Keywords
This publication has 14 references indexed in Scilit:
- Characterization of the effects of AHN 086, an irreversible ligand of "peripheral" benzodiazepine receptors, on contraction in guinea-pig atria and ileal longitudinal smooth muscleCanadian Journal of Physiology and Pharmacology, 1989
- Effects of sodium nitroprusside on cytosolic calcium level in vascular smooth muscleEuropean Journal of Pharmacology, 1988
- Bay K 8644‐induced changes in the ECG pattern of the rat and their inhibition by antianginal drugsBritish Journal of Pharmacology, 1987
- Binding of [3H]Ro 5–4864 and [3H]PK 11195 to Cerebral Cortex and Peripheral Tissues of Various Species: Species Differences and Heterogeneity in Peripheral Benzodiazepine Binding SitesJournal of Neurochemistry, 1987
- Benzodiazepines and calcium channel functionTrends in Pharmacological Sciences, 1986
- PK 11195, an antagonist of peripheral type benzodiazepine receptors, modulates BAY K8644 sensitive but not β- or H2-receptor sensitive voltage operated calcium channels in the guinea pig heartLife Sciences, 1986
- Pharmacological evidence that the non-neuronal diazepam binding site in primary cultures of glial cells is associated with a calcium channelEuropean Journal of Pharmacology, 1985
- Electrophysiological and pharmacological evidence that peripheral type benzodiazepine receptors are coupled to calcium channels in the heartLife Sciences, 1985
- Histidine modification with diethylpyrocarbonate induces a decrease in the binding of an antagonist, PK 11195, but not of an agonist, R05–4864, of the peripheral benzodiazepine receptorsLife Sciences, 1984
- Characterization of the Binding of [3H]Ro 5‐4864, a Convulsant Benzodiazepine, to Guinea Pig BrainJournal of Neurochemistry, 1984