Staining Nerve Fibers with Silver in Tissue Fixed with Bouin's Fluid

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Abstract
Nerve fibers, in organs fixed with Bouin''s fluid, are usually refractive to the Davenport Ag technic. The axons, however, can be successfully stained if the sections, on slides, are given a preliminary treatment with conc. pyridine (1 hr.), then a 24-hr. bath of ammoniated alc. (99 cc. 80% alc. 1 cc. 28% NH4OH) and an interval in 40% aq. AgNO3 (6-8 hrs.) before being immersed in the acidified alcoholic Ag soln. of Davenport. Following the silvering, reduction and toning of the axons, according to the procedure of Davenport, the surrounding non-nervous tissue elements can be counterstained with a combination of either azocarmine, light green and orange G, or azocarmine, aniline blue and orange G.

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