Promoter switching during development and the termination site of the σ43 operon of Bacillus subtilis

1 April 1987

journal article
research article
Published by Springer Nature in Molecular Genetics and Genomics

Vol. 207 (1) , 114-119
https://doi.org/10.1007/bf00331498

Abstract

Sequencing data indicated that the RNA polymerase (σ⁴³) operon of Bacillus subtilis consisted of three genes, P23 (function unknown), dnaE (DNA primase), and rpoD (σ⁴³) (Wang and Doi 1986a). S1 nuclease mapping experiments with RNA from various stages of growth demonstrated the presence of two overlapping σ⁴³ promoters that controlled the expression of the operon during growth and a σ³⁷ promoter that regulated the expression of the operon during the sporulation phase. This promoter switching mechanism ensured that this important operon would be expressed during different nutritional states of the cell and also illustrated a function for the minor RNA polymerase σ³⁷ holoenzyme in the expression of genes which are normally expressed during the logarithmic phase of growth. The location of the transcription termination signal confirmed that the σ⁴³ operon consists of three genes.

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