Rapid virological surveillance of community influenza infection in general practice

Abstract
Six practices took part. Influenza-like illness was defined by using standard criteria. Combined nose and throat swabs were submitted in both lysis buffer3 and viral transport medium. Two serum samples were taken a minimum of three weeks apart. All samples were posted to the laboratory. Influenza A and B reverse transcription PCR was performed on both media.3 Primary rhesus monkey kidney cells (Biowhittaker, Wokingham) were used to isolate virus. Influenza A and B antibodies were measured using the complement fixation test.

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