Kirromycin‐resistant elongation factor Tu from wild‐type of Lactobacillus brevis
- 20 September 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 146 (2) , 322-326
- https://doi.org/10.1016/0014-5793(82)80944-7
Abstract
Properties of the elongation factor Tu from Lactobacillus brevis which is naturally insensitive to kirromycin are described. The protein is characterized by an unusual nucleotide‐binding site with increased affinity for GTP and extreme heat stability. EF‐Tu is sensitive to pulvomycin in the assay of polyphenylalanine synthesis. However, the failure of the protein to display pulvomycin‐dependent GDP‐binding and GTPase activity indicates that pulvomycin action in L. brevis differs from that in E. coli.Keywords
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