Simultaneous demonstration of infecting fungi and DNA‐synthesizing epithelial cells in the skin
- 1 July 1993
- Vol. 36 (7-8) , 267-269
- https://doi.org/10.1111/j.1439-0507.1993.tb00763.x
Abstract
An excellent staining method to demonstrate in vivo dermatophytes and DNA-synthesizing cells in the skin is described. Deparaffinized sections of guinea pig skin, infected with dermatophytes and given bromodeoxyuridine (BrdU) by intraperitoneal injection, were used. Periodic acid-Schiff (PAS) stain in combination with an immunohistochemical stain using anti-BrdU monoclonal antibody allowed simultaneous visualization of fungi and DNA-synthesizing epithelial cells in the skin. Many epithelial cells near the infecting fungi in follicles and interfollicular skin were labelled with BrdU. Only a few BrdU-positive cells were observed in the skin of uninfected animals.Keywords
This publication has 5 references indexed in Scilit:
- Cell kinetic study of human and mouse hair tissues using anti-bromodeoxyuridine monoclonal antibodyJournal of Dermatological Science, 1990
- Inflammation and Immunity in DermatophytosisDermatology, 1989
- Experimental tinea pedis induced by non-abrasive inoculation ofTrichophyton mentagrophytesarthrospores on the plantar part of a guinea pig footMedical Mycology, 1987
- Epidermal Cell Proliferation in Guinea Pigs with Experimental DermatophytosisJournal of Investigative Dermatology, 1985
- Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA ReplicationScience, 1982