Zytochemische Untersuchungen zur Entwicklung der grossen mononukleären Zellen des Hautfensters

Abstract
Various cytochemical tests of the activity of unspecific esterase, chlor-acylesterase, acid phosphatase, peptidase and alkaline phosphatase, and fat staining with Sudan black B were carried out on skin-window preparations. The marked activity of the large mononucleocytes and giant cells in the naphthol AS-acetate esterase was almost completely blocked in both cases by the addition of 1.5 mg/ml sodium fluoride to the incubation solution. These cells were then comparable to the monocytes in the blood. The considerable increase in hydrolytic enzymes at a slightly acid pH, and their primary location in the Golgi zone, indicates an increase in lysosome in the mononucleocytes.

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