COMPARATIVE BIOASSAY OF PROSTAGLANDIN E2 AND ITS THREE PULMONARY METABOLITES

Abstract
The relative potencies of prostaglandin E2 and its metabolites 15‐keto PGE2, 13,14‐dihydro‐15‐keto‐PGE2 and 13,14, dihydro‐PGE2 were investigated on isolated smooth muscle preparations. These were rat stomach strip, colon and uterus, chick rectum, guinea‐pig ileum, trachea and pulmonary artery and rabbit aorta and pulmonary artery. 15‐keto PGE2 was equiactive or 1–1.8 times more potent than prostaglandin E2 in relaxing guinea‐pig trachea but otherwise the three metabolites were less active than prostaglandin E2 on all preparations.