Abstract
Allozymes at several polymorphic loci were assayed in larval collections of 12 recognized species and two possible new species ofChoristoneuraand two species ofArchips. Most of the 48 collections came from high density populations, and those ofC.fumiferana,C.occidentalis, andC.pinusrepresented much of the geographic range of these species. Mean percentage heterozygosity ranged from 2.0 to 18.6%, based on nine polymorphic loci. Three loci are sex-linked inC.fumiferana, two inC.pinusandC.occidentalisand probably in some other members of the group. Allozymes of aspartate transaminase (AAT-1) were most varied among the species and permit identification of individualC.fumiferanain better than 95% of cases. Among the group of coniferophagousChoristoneuraspecies genetic distances were small (max. Nei = 0.232);C.fumiferanawas the most distinct species. Wagner trees based on modified Rogers’ distances supported the above conclusions but indicated that separations amongC.biennis,C.orae,C.occidentalis,C.carnana,C.subretiniana, and the two new species ofChoristoneurawere very small and probably below the species level, based on the allozymes measured.

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