The guinea pig tracheobronchial spiral strip: responses to selected agonists

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Abstract
The guinea pig tracheal spiral strip is a useful preparation for studying bronchoconstrictor and bronchodilator compounds. Employing a simple and rapid modification of this technique, experiments were performed in vitro to quantitate the effects of selected bronchospastic agents on guinea pig tracheobronchial smooth muscle. An upper tracheal, a lower tracheal and a bronchial segment of the main airways were prepared from each animal. The dose-dependent contractile responses of the 3 tissue segments were determined for carbachol, acetylcholine, histamine, 5-hydroxytryptamine, and bradykinin. Differences were observed amongst the agonists in magnitudes of contraction, effective concentration ranges and slopes of dose-response curves. ED25, ED50, and ED75 values were calculated from regression analysis of dose-response data. The relative order for these agents to produce maximum contractions was carbachol .simeq. acetylcholine > histamine > 5-hydroxytryptamine > bradykinin. There was no significant difference between the 3 tissue segments in their responses to the various agonists.

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