ENDONUCLEASE FINGERPRINTING OF PLASMIDS MEDIATING GENTAMICIN RESISTANCE IN AN OUTBREAK OF HOSPITAL INFECTIONS
- 1 August 1980
- journal article
- research article
- Published by Wiley in Immunology & Cell Biology
- Vol. 58 (4) , 331-338
- https://doi.org/10.1038/icb.1980.32
Abstract
Three plasmids which each determined the same extensive antibiotic resistance phenotype, including resistance to gentamicin, and which had been recovered from organisms involved in an outbreak of gentamicin-resistant infections in Melbourne hospitals in 1975–1976, were analysed by fingerprinting with the restriction endonucleases Eco RI, Hind III and Pst I. In each case the fingerprints for all three plasmids were identical, despite the tact that each plasmid originated in a different host species at different hospitals. This observation confirms our earlier hypothesis (Davey and Pittard, 1977) that the plasmids mediating gentamicin resistance in the outbreak were the closely related descendants of one ancestral(IncL) plasmid.Keywords
This publication has 4 references indexed in Scilit:
- Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.Journal of Bacteriology, 1978
- PLASMIDS MEDIATING RESISTANCE TO GENTAMICIN AND OTHER ANTIBIOTICS IN ENTEROBACTERIACEAE FROM FOUR HOSPITALS IN MELBOURNEImmunology & Cell Biology, 1977
- Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gelsJournal of Molecular Biology, 1977
- Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acidJournal of Bacteriology, 1976