Elicitor-stimulated ion fluxes and O 2 − from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley
Open Access
- 29 April 1997
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 94 (9) , 4800-4805
- https://doi.org/10.1073/pnas.94.9.4800
Abstract
Fungal elicitor stimulates a multicomponent defense response in cultured parsley cells (Petroselinum crispum). Early elements of this receptor-mediated response are ion fluxes across the plasma membrane and the production of reactive oxygen species (ROS), sequentially followed by defense gene activation and phytoalexin accumulation. Omission of Ca2+ from the culture medium or inhibition of elicitor-stimulated ion fluxes by ion channel blockers prevented the latter three reactions, all of which were triggered in the absence of elicitor by amphotericin B-induced ion fluxes. Inhibition of elicitor-stimulated ROS production using diphenylene iodonium blocked defense gene activation and phytoalexin accumulation. O2− but not H2O2 stimulated phytoalexin accumulation, without inducing proton fluxes. These results demonstrate a causal relationship between early and late reactions of parsley cells to the elicitor and indicate a sequence of signaling events from receptor-mediated activation of ion channels via ROS production and defense gene activation to phytoalexin synthesis. Within this sequence, O2− rather than H2O2 appears to trigger the subsequent reactions.Keywords
This publication has 54 references indexed in Scilit:
- Calcium-mediated apoptosis in a plant hypersensitive disease resistance responseCurrent Biology, 1996
- Activation of defense-related genes in parsley leaves by infection withErwinia chrysanthemiEuropean Journal of Plant Pathology, 1995
- Roles of Ion Channels in Initiation of Signal Transduction in Higher Plants.Plant Cell, 1995
- Roles of Ion Channels in Initiation of Signal Transduction in Higher PlantsPlant Cell, 1995
- H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance responseCell, 1994
- Temporal and Spatial Patterns of Gene Expression around Sites of Attempted Fungal Infection in Parsley LeavesPlant Cell, 1989
- Chloride channels in epitheliaBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1988
- Estimating viability of plant protoplasts using double and single stainingProtoplasma, 1986
- Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic FungiPlant Physiology, 1986
- The isolation of nuclei from tissue-cultured plant cellsExperimental Cell Research, 1981