Affinity Chromatography and Characterization of the Acetylcholine Receptor from Torpedo californica
- 1 January 1976
- journal article
- research article
- Published by Taylor & Francis in Journal of Macromolecular Science: Part A - Chemistry
- Vol. 10 (1-2) , 73-109
- https://doi.org/10.1080/00222337608068092
Abstract
Affinity chromatography has become one of the most important tools available to the biochemist for the selective isolation and purification of- interesting biological macromolecules. pioneering work of Porath [1,2] and subsequent developmental work by Porath [33 and Cuatrecasas 141 have served as practical guides for the successful application of affinity chromatography in many The areas of biochemical research. Specific ligands can be covalently attached to an insoluble, stationary support such as agarose or polyacrylamide beads and used to “fish out” those components which show an appreciable affinity for the ligands.Keywords
This publication has 41 references indexed in Scilit:
- Preferential chemical modification of a binding subsite on the acetylcholine receptorBiochemical and Biophysical Research Communications, 1975
- The acetylcholine receptor: Progress reportLife Sciences, 1974
- The nicotinic cholinergic receptor protein: improved purification method, preliminary amino acid composition and observed auto‐immuno responseJournal of Neurochemistry, 1974
- Use of a fluorescent probe for the study of ligand binding by the isolated cholinergic receptor of Torpedo californicaBiochemical and Biophysical Research Communications, 1973
- Molecular properties of the cholinergic receptor purified from Electrophorus electricusBiochemistry, 1973
- Biochemical studies on torpedo californica acetylcholine receptorsJournal of Supramolecular Structure, 1973
- Demonstration of a specific α-bungarotoxin binding component in Electrophorus electricus electroplax membranesBiochemical and Biophysical Research Communications, 1971
- Amino acid sequence of α-bungarotoxin from the venom of bungarus multicinctusBiochemical and Biophysical Research Communications, 1971
- The “Desensitizing” Effect of Acetylcholine on the Mammalian Motor End-Plate.Acta Physiologica Scandinavica, 1958
- Methods to estimate the turnover number of preparations of ox red cell cholinesteraseBiochimica et Biophysica Acta, 1953