Evidence for N‐formyl chemotactic peptide‐stimulated GTPase activity in human neutrophil homogenates
- 23 January 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 166 (1) , 165-169
- https://doi.org/10.1016/0014-5793(84)80065-4
Abstract
Neutropil homogenates contained a high affinity guanosine triphosphatase (GTPase) that was stimulatable (+ 27%) by the addition of 100 nM N-formyl chemotactic peptide (CHO-pep), but not by 1 μg·ml−1 phorbolmyristate acetate (PMA). Kinetic analysis of the stimulation demonstrated an apparent lagtime of 14.3 ± 6.9 s between the addition of CHO-pep and the optimal GTPase stimulation. The GTPase activity (but not CHO-pep-stimulated GTPase activity) was preserved in a highly purified plasma membrane fraction of the homogenate. From these observations we suggest that both a high affinity guanine nucleotide binding protein and GTPase are closely associated with the plasma membrane CHO-pep receptor. The possibility that GTPase activity may influence guanine nucleotide regulation of adenylate cyclase during CHO-pep stimulation of neutrophils is discussed.Keywords
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