Structural Elucidation on Succinoglycan and Related Polysaccharides fromAgrobacteriumandRhizobiumby Fragmentation with Two Special β-d-Glycanases and Methylation Analysis

Publisher Website
Google Scholar
Abstract
The structure of the extracellular acidic polysaccharide succinoglycan from Alcaligenes faecalis var. myxogenes 10C3 was elucidated by successive fragmentation of the polysaccharide with extracellular β-d-glycanase (succinoglycan depolymerase) and intracellular endo-β-(1→6)-d-glucanase of Flavobacterium sp. M64 into two tetrasaccharides via its octasaccharide repeating unit, and then methylation analysis and enzymic hydrolysis of the products. The extracellular acidic polysaccharides from Rhizobium meliloti, Agrobacterium radiobacter, Agrobacterium rhizogenes and Agrobacterium tumefaciens were also analyzed by this method and found to be identical with the polysaccharide from Alcaligenes faecalis var. myxogenes, irrespective of their modes of acylation. The structure was identical with that of extracellular polysaccharide from Rhizobium meliloti presented by Jansson et al. (J. Am. Chem. Soc., 99, 3812 (1977)), except for the occurrence of the succinic acid residue.