Effect of Interaction of Heavy Metals on (Na+‐K+) ATPase and the Uptake of 3H‐DA and 3H‐NA in Rat Brain Synaptosomes
- 13 March 1984
- journal article
- research article
- Published by Wiley in Acta Pharmacologica et Toxicologica
- Vol. 54 (3) , 210-213
- https://doi.org/10.1111/j.1600-0773.1984.tb01919.x
Abstract
The effect of interaction of Mn2+, Pb2+ and Cd2+ on Na+,K+-ATPase and uptake of labeled dopamine (3H-DA) and labeled noradrenaline [norepinephrine] (3H-NA) were studied in vitro in rat brain synaptosomes. The inhibition of Na+,K+-ATPase by Pb2+ and Cd2+ alone was concentration dependent; Mn2+ had almost no effect on the activity of this enzyme. Interaction of Cd2+ with Pb2+ or Mn2+ was most powerful in inhibiting the activity of synaptosomal transport ATPase. Lower concentrations of Pb2+ increased while higher concentrations inhibited synaptosomal uptake of 3H-DA and 3H-NA. Lower concentrations of Cd2+ increased the uptake of 3H-DA while at concentrations of 100 .mu.M, the uptake was inhibited; this metal had strong inhibitory effect on the uptake of 3H-NA. Mn2+ inhibited the uptake of labeled amines. Interaction Mn2+ with Pb2+ or Cd2+ produced inhibition on the uptake of 3H-DA and 3H-NA. The results of the uptake of biogenic amines in the presence of metal ions apparently had no correlation with the activity of Na+,K+-ATPase which was involved in the active transport of cations across cell membranes.Keywords
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