Development of a bifunctional crosslinking agent with potential for the preparation of immunotoxins
- 1 October 1988
- journal article
- Published by Springer Nature in Protein Journal
- Vol. 7 (5) , 581-592
- https://doi.org/10.1007/bf01024876
Abstract
A new protein crosslinking agent, 2,3-dibromopropionyl-N-hydroxysuccinimide ester, has been synthesized and characterized. The potential use of this compound as a temperature-controllable heterobifunctional crosslinking agent has been investigated using model systems and its reactivity compared with that of chlorambucil-N-hydroxysuccinimide ester. The coupling of14C-labeled phenylethylamine to lysozyme has been used to illustrate the feasibility of the use of this crosslinking agent for the synthesis of immunotoxins.Keywords
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