HISTOCHEMISTRY OF LYSOSOMES IN RAT HEART MUSCLE

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Abstract
Rat heart muscle was studied histochemically by the naphthol and lead methods for hydrolases (acid phosphatase, esterase, β-glucuronidase and aryl sulfatase). Activity of the enzymes studied is demonstrable in granules which by their staining properties are identified as lysosomes. These organelles are usually perinuclear in location and display variable amounts of enzyme activity. The heart lysosomes in chloroquine-treated rats are enlarged and aggregated together. The effects of the detergent Triton X-100 on the lysosomal hydrolases are discussed.