Physiological and Morphogenetic Studies of Fern Gametophyte by Aseptic Culture

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Abstract
Callus formation could be induced in young gametophytes of P. vittata by culturing in the dark on a complex liquid medium. The callus tissue obtained was either friable or compact. Friable callus tissue placed in liquid medium in a test tube with agitation produced small pieces of tissue, and a suspension consisting of cell aggregates and isolated cells. When the suspension was distributed on the surface of the complex medium, large masses of callus tissue reformed. However, when the suspension was placed on a defined medium devoid of organic substances gametophytes were differentiated. These gametophytes developed to maturity with the formation of sex organs and sporophytes from cell aggregates or directly from single cells.