Identification of Enterohemorrhagic Escherichia coli O26:H − Genes Required for Intestinal Colonization in Calves
Open Access
- 1 March 2005
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 73 (3) , 1735-1743
- https://doi.org/10.1128/iai.73.3.1735-1743.2005
Abstract
Enterohemorrhagic Escherichia coli (EHEC) infections in humans are an important public health problem and are commonly acquired via contact with ruminant feces. The serogroups that are predominantly associated with human infection in the United States and Europe are O157 and O26. Serotypes O157:H7 and O26:H− differ in their virulence and tissue tropism in calves and therefore may colonize calves by distinct mechanisms. The mechanisms underlying EHEC intestinal colonization and pathogenesis are poorly understood. Signature-tagged mutagenesis was used to identify 59 genes of EHEC O26:H− that are required for the intestinal colonization of calves. Our results indicate important roles for locus of enterocyte effacement (LEE)-encoded type III secreted proteins in intestinal colonization. In addition, colonization is facilitated by cytotoxins, putative type III secreted proteins unlinked to the LEE, a putative fimbrial operon, and numerous genes involved in central metabolism and transport and genes of unknown function. Our data also imply that the elaboration of type I fimbriae by EHEC O26:H− is disadvantageous for persistence within the bovine intestines. These observations have important implications for the design of vaccines to control these important zoonotic pathogens.Keywords
This publication has 80 references indexed in Scilit:
- Glucoamylase‐like domains in the α‐ and β‐subunits of phosphorylase kinaseProtein Science, 2003
- Distribution of the Secondary Type III Secretion System Locus Found in Enterohemorrhagic Escherichia coli O157:H7 Isolates among Shiga Toxin-Producing E. coli StrainsJournal of Clinical Microbiology, 2003
- Functional Comparison of Serine Protease Autotransporters of EnterobacteriaceaeInfection and Immunity, 2002
- Role of Non‐O157:H7Escherichia coliin Hemolytic Uremic SyndromeClinical Infectious Diseases, 2002
- Attaching and effacing lesions in the large intestine of an eight‐month‐old heifer associated with Escherichia coli 026 infection in a group of animals with dysenteryVeterinary Record, 1999
- Serodiagnosis of infection with Verocytotoxin‐producingEscherichia coliJournal of Applied Microbiology, 1999
- Induction and Evasion of Host Defenses by Type 1-Piliated Uropathogenic Escherichia coliScience, 1998
- Mast cell modulation of neutrophil influx and bacterial clearance at sites of infection through TNF-αNature, 1996
- Simultaneous Identification of Bacterial Virulence Genes by Negative SelectionScience, 1995
- Hemorrhagic Colitis Associated with a RareEscherichia coliSerotypeNew England Journal of Medicine, 1983