Recovery of Colony-Forming Ability of X-Irradiated L Cells by Cell Fusion
- 1 July 1976
- journal article
- letter
- Published by JSTOR in Radiation Research
- Vol. 67 (1) , 178-183
- https://doi.org/10.2307/3574509
Abstract
Neither mouse fibroblast L cells irradiated with 3-20 krad of X-rays, nor 8-azaguanine-resistant cells, produced colonies when grown in HAT [hypoxanthine and thymine] selective media. If, however, 106 irradiated cells were mixed with the same number of 8-azaguanine-resistant L cells and UV-inactivated HVJ [hemagglutinating virus of Japan (Sendai virus)] virus which promotes cell fusion, colonies were always observed. In multiple experiments, between 6 and 53 colonies were counted. Chromosome numbers of 2 clones isolated following hybridization of 8-azaguanine-resistant cells with L cells irradiated with 5 krad were 68 and 86; chromosome numbers of 4 clones isolated following 20-krad exposure were 54, 54, 56 and 60. Thus, the higher the X-irradiation dose, the smaller the chromosome numbers in hybrid cells.This publication has 2 references indexed in Scilit:
- Hybridization of dwarf hamster cells by UV-inactivated Sendai virus.Proceedings of the National Academy of Sciences, 1966
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964