Effect of Heat-Induced Susceptibility of Tobacco to Black Shank on Protein Content and on Activity of Peroxidases

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Abstract
When tobacco [Nicotiana tabacum] plants of cultivars Coker 187 (resistant) and Virginia Gold (susceptible) were immersed in a water bath at 50.degree. C for 1 min and inoculated with Phytophthora parasitica var. nicotianae, disease severity of both cultivars was increased up to 100% compared with nontreated controls. In root tissue, the protein concentration significantly decreased as disease severity increased in both cultivars. Disease susceptibility was not correlated with levels of peroxidase activity in extracts from both cultivars. The heat treatment, however, induced 4 distinct peroxidase bands in noninoculated and inoculated ''Coker 187'' roots after 1 h and 10 days, as shown by disc electrophoresis. These bands were not present in gels of nonheated ''Coker 187'' root extracts.

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