Use of synthetic gramicidins in the determination of channel structure and mechanism
- 12 January 1983
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 21 (1) , 16-23
- https://doi.org/10.1111/j.1399-3011.1983.tb03073.x
Abstract
The syntheses of (1-13C) Trp9 gramicidin A (GA), (1-13C) Trp11 GA, (1-13C) Trp13 GA, (1-13 C) Trp15 GA and D-Leu2 GA are verified by means of high performance liquid chromatography, 13C NMR, circular dichroism and characterization of transport properties. The use of these and other synthetic gramicidins is discussed in terms of determining ion binding sites within the channel, helix sense of the channel, the basis of monovalent vs. divalent cation selectivity, and means of modulating channel conductance.Keywords
This publication has 11 references indexed in Scilit:
- Ion interactions in (1-13C)d-Val8 andd-Leu14 analogs of gramicidin A, the helix sense of the channel and location of ion binding sitesThe Journal of Membrane Biology, 1982
- Synthesis and characterization of 1‐13C‐DLeu12,14 gramicidin AInternational Journal of Peptide and Protein Research, 1982
- Location of monovalent cation binding sites in the gramicidin channel.Proceedings of the National Academy of Sciences, 1982
- Single channel properties of d-Leu2-gramicidin A side chain modulation of channel lifetimeBiochimica et Biophysica Acta (BBA) - Biomembranes, 1981
- Gramicidin forms multi-state rectifying channelsNature, 1981
- Conduction processes of the gramicidin channelInternational Journal of Quantum Chemistry, 1981
- Rate theory calculation of gramicidin single-channel currents using NMR-derived rate constants.Proceedings of the National Academy of Sciences, 1980
- Characterization of micellar-packaged gramicidin a channelsBiochemical and Biophysical Research Communications, 1979
- Channel Structures of Gramicidin: Characterization of Succinyl DerivativesScience, 1978
- Solid Phase Peptide Synthesis. I. The Synthesis of a TetrapeptideJournal of the American Chemical Society, 1963