Biochemical Transformation of Mouse Cells by a Purified Fragment of Marmoset Herpesvirus DNA
- 1 January 1980
- journal article
- research article
- Published by S. Karger AG in Intervirology
- Vol. 13 (2) , 110-121
- https://doi.org/10.1159/000149115
Abstract
Although the size of marmoset herpesvirus (MarHV) DNA, estimated by velocity sedimentation in sucrose gradients, was similar to that of herpes simplex virus type 1 (HSV-1) DNA, the restriction endonuclease sites of MarHV and HSV-1 DNAs were quite different. A specific Bam HI restriction fragment (6.2 × 106 daltons) of MarHV DNA biochemically transformed LM(TK–) mouse fibroblasts to the thymidine kinase(TK)-positive phenotype. Rabbit antisera, prepared against MarHV TK, inhibited MarHV-induced TK, but not HSV-1, HSV-2, or cellular TKs. Disc PAGE analyses and enzyme neutralization experiments with the anti-MarHV TK sera demonstrated that the TK expressed in MarHV transformants was MarHV-specific.This publication has 16 references indexed in Scilit:
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