Differential effect of secondary aqueous humor from rabbit and cat on DNA synthesis of cultured bovine corneal endothelial cells

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Abstract
The addition of medium containing secondary aqueous humor (2°AH) from either cat or rabbit eyes to quiescent preconfluent cultures of bovine corneal endothelial cells resulted in an increase in the incorporation of 3H-thymidine into DNA. This increase in DNA synthesis in response to 2°AH was dose-dependent and was significantly greater than in control cultures maintained in medium containing 1% bovine calf serum alone. The 2°AH of both cat and rabbit was found to contain elevated levels of protein and 6-keto-prostaglandin-F1α but not thromboxane B2. The proliferative response of bovine cells induced by rabbit 2°AH was significantly greater than the response induced by cat 2°AH containing equivalent amounts of aqueous protein.