A comparison of DNA cleavage by the restriction enzymes SalPI and PstI
Open Access
- 11 November 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (21) , 4943-4954
- https://doi.org/10.1093/nar/8.21.4943
Abstract
Methods for obtaining highly active, exonuclease-free, stable preparations of the Streptomyces albus P restriction enzyme SalPI are described. SalPI and its isoschizomer PstI (from the taxonomically distant Providencia stuartii 164) both cleave their recognition sequence (5′-CTGCAG-3′) to generate fragments terminating in tetranucleotide 3′ extensions whose sequence is 5′-TGCA-3′. Bacteriophage R4G2 DNA, protected against SalPI cleavage by pregrowth on S. albus P, is also protected against PstI cleavage; and total DNA of both S. albus P and P. stuartii 164 is resistant to cleavage by both enzymes.Keywords
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