HETEROGENEITY OF HUMAN COLONY-FORMING CELLS (CFU-C) - DIFFERENCES IN SIZE, RATE OF COLONY FORMATION, AND RESPONSIVENESS TO COLONY-STIMULATING FACTOR

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 92  (1) , 38-44
Abstract
Human bone marrow cells were fractionated by velocity sedimentation at unit gravity. Fractions were analyzed for cell morphology, number of nucleated cells and myeloid CFU-C. Colony formation was assayed with the following CSF [colony-stimulating factor] preparations: serum-free HLCM [human long-conditioned medium] and 2 electrophoretically distinct CSF fractions (CSF-A and CSF-B) purified from the same source. Two distinct CFU-C populations were found. One, a rapidly sedimenting (7.2-8.0 mm/h), population exhibited colonies after 7 days of culture in response to HLCM and CSF-A only. The 2nd, a more slowly sedimenting (6.5 mm/h) CFU-C peak, did not exhibit colonies until 11 days of culture and did so in response to all 3 CSF tested. Human bone marrow CFU-C are apparently heterogeneous, and the 2 purified CSF fractions from human lung apparently have different CFU-C specificity.