Phorbol ester treatment impairs hormone‐ but not stable GTP analog‐induced inhibition of adenylate cyclase
- 17 March 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 198 (1) , 43-46
- https://doi.org/10.1016/0014-5793(86)81181-4
Abstract
Treatment of intact human platelets and S49 lymphoma cyc− cells with the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate, impairs GTP-dependent and hormone-induced inhibition of adenylate cyclase, an action mediated by the inhibitory coupling protein Ni. In contrast, receptor-independent activation of Ni with subsequent adenylate cyclase inhibition induced by the stable GTP analog, guanosine 5'-[γ-thio]triphosphate, was affected in neither the potency nor onset of Ni activation by the stable GTP analog, in both membrane systems studied. The data indicate that modification of Ni following phorbol ester treatment does not impair its activation by stable GTP analogsKeywords
This publication has 16 references indexed in Scilit:
- Protein kinase C interferes with Ni‐mediated inhibition of human platelet adenylate cyclaseFEBS Letters, 1985
- Molecular Cloning of Complementary DNA for the Alpha Subunit of the G Protein that Stimulates Adenylate CyclaseScience, 1985
- Protein kinase C phosphorylates the inhibitory guanine‐nucleotide‐binding regulatory component and apparently suppresses its function in hormonal inhibition of adenylate cyclaseEuropean Journal of Biochemistry, 1985
- Modulation of adenylate cyclase of human platelets by phorbol esterEuropean Journal of Biochemistry, 1985
- Mechanism of pertussis toxin action on the adenylate cyclase systemEuropean Journal of Biochemistry, 1984
- Adenylate cyclase inhibition and GTPase stimulation by somatostatin in S49 lymphoma cyc− variants are prevented by islet‐activating proteinFEBS Letters, 1983
- Synergistic inhibition of human platelet adenylate cyclase by stable GTP analogs and epinephrineBiochimica et Biophysica Acta (BBA) - Biomembranes, 1983
- Islet‐activating protein prevents nicotinic acid‐induced GTPase stimulation and GTP but not GTPγS‐induced adenylate cyclase inhibition in rat adipocytesFEBS Letters, 1983
- Stimulation and inhibition of adenylyl cyclases mediated by distinct regulatory proteinsNature, 1983
- Occurrence of an Inhibitory Guanine Nucleotide‐Binding Regulatory Component of the Adenylate Cyclase System in cyc− Variants of S49 Lymphoma CellsEuropean Journal of Biochemistry, 1983