Improved Gas-Liquid Chromatographic Method for Determining Ethylenethiourea in Plants
Open Access
- 1 September 1974
- journal article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 57 (5) , 1015-1021
- https://doi.org/10.1093/jaoac/57.5.1015
Abstract
In the improved method for determining ethylenethiourea (ETU) in plants, pentafluorobenzoyl chloride was substituted for trifluoroacetic anhydride derivatization of S-benzylated ETU. The resulting product (2-(benzylthio)-l- (pentafluorobenzoyl)-2-imidazoline) gave recoveries of near 100% and improved the electron capture gas-liquid chromatographic (GLC) response. The product can be cleaned up by column chromatography to eliminate interfering GLC peaks. Soybeans grown in soil treated with 14C-ETU resulted in a high concentration of 14C residues in leaves, but only traces of ETU were detected by either 14C or GLC measurements.Keywords
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