Analysis of Cellular Responses to Native and Recombinant Proteins of Cowdria ruminantiumaa
- 1 June 1998
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 849 (1) , 155-160
- https://doi.org/10.1111/j.1749-6632.1998.tb11045.x
Abstract
The response of PBMC collected from cattle immunized with killed Cowdria to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and gamma delta T cells proliferated in response to recombinant proteins. Short-term cell lines derived from PBMC using Cowdria lysates were more than 90% CD4+ and did not respond to any of the recombinant proteins. Restimulation of PBMC with MAP1, MAP1-B, and MAP2 proved difficult, and cultures usually stopped proliferating after 2 to 3 weeks. Only one cell line could be generated using MAP1 and it was composed of 85% gamma delta T cells. This study indicates that in animals immunized with killed Cowdria, proteins other than the serologically immunodominant MAP1 and MAP2 are strongly immunogenic for T lymphocytes.Keywords
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