Preparation of delipidized serum protein for use in cell culture systems
- 1 August 1976
- journal article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology - Plant
- Vol. 12 (8) , 554-557
- https://doi.org/10.1007/bf02797438
Abstract
A rapid procedure for the preparation of delipidized serum protein is described. The delipidized protein can be used for the maintenance and growth of tissue culture cells in a lipid-free environment. The extraction procedure greatly reduces all serum lipid classes and the delipidized protein supports the growth of a variety of cells in culture.Keywords
This publication has 18 references indexed in Scilit:
- Cholesteryl ester metabolism in tissue culture cells. I. Accumulation in Fu5AH rat hepatoma cellsLipids, 1974
- Regulation of cellular sterol flux and synthesis by human serum lipoproteinsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1974
- Studies of sterol synthesis, synthesis regulation and transport in cultured human kidneyExperimental Cell Research, 1974
- Lipids of cultured hepatoma cells: I. Effect of serum lipid levels on cell and media lipidsLipids, 1973
- Quantitation of serum lipids by a simple tlc-charring methodClinica Chimica Acta; International Journal of Clinical Chemistry, 1973
- Cholesterol biosynthesis in WI-38 and WI-38VA13A tissue culture cellsExperimental Cell Research, 1971
- Desmosterol as the Major Sterol in L-Cell Mouse Fibroblasts Grown in Sterol-Free Culture MediumScience, 1970
- CHOLESTEROL REQUIREMENT OF PRIMARY DIPLOID HUMAN FIBROBLASTSThe Journal of cell biology, 1969
- Control of Growth of Axenic Cultures of Mammalian Cells by Serum LipidsNature, 1968
- Separation and characterization of the protein moiety of human α1-lipoproteinArchives of Biochemistry and Biophysics, 1958