Rapid Identification of Staphylococcus aureus and the mecA Gene from BacT/ALERT Blood Culture Bottles by Using the LightCycler System
Open Access
- 1 July 2002
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (7) , 2659-2661
- https://doi.org/10.1128/jcm.40.7.2659-2661.2002
Abstract
One hundred BacT/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the sa442 and mecA genes. PCR was 100% sensitive and specific for detecting Staphylococcus aureus and methicillin resistance in S. aureus but was less accurate for methicillin resistance in coagulase-negative staphylococci.Keywords
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