Minor and trace sterols in marine invertebrates. Part 35. Isolation and structure elucidation of seventy-four sterols from the sponge Axinella cannabina

Abstract
Seventy-four 3β-hydroxy-sterols, of which twenty-four are new, with seven different nuclei [Δ5,7,9(11), Δ5,7, Δ8, Δ7, Δ5, 5α-saturated; and 5α-methoxy-Δ6,8(14)], and with eighteen different C7–C10-side chains, have been isolated by reverse-phase h.p.l.c. and argentic t.l.c. from the marine sponge Axinella cannabina. Characterization was accomplished by g.l.c.–m.s., high-resolution m.s., and 360-MHz 1H n.m.r. and u.v. spectroscopy.

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