The effect of phencyclidine on [3H]GABA and [3H]fulnitrazepam binding in the brain of naive and handling‐habituated rats
- 1 January 1995
- journal article
- Published by Wiley in Acta Physiologica Scandinavica
- Vol. 153 (1) , 33-41
- https://doi.org/10.1111/j.1748-1716.1995.tb09831.x
Abstract
The effects of handling and handling combined with phencyclidine (PCP) treatment on GABAergic neurotransmission were studied in Sprague‐Dawley rats. The animal material consisted of handling‐habituated (HH, for 11 d), acutely handled (naive, N), handling‐habituated and PCP‐treated (10 mg kg‐1 i. p., HH+PCP) and acutely handled (naive) PCP‐treated (N+PCP) and unhandled ‘control’ rats. The binding of [3]GANA and [3H]flunitrazepam (FLU) was studied with membrances and the release of [3H]GABA with slices prepared from the striatum and frontal cortex. In the striatum the maximal binding capacity (Bmax) and the binding constant (KD was lower in N rats. KD constants of [3H]FLU were significantly lower in both brain areas in N rats than in HH rats. After PCP treatment both Bmax and KD for [3H]GAGA diminished. Neither handlign nor PCP had any effect on [3H]GABA release from striatal and frontal cortical slices. Handling prior to killing thus affects differently the GABAergic parameters studied and modulates the PCP‐induced effectsKeywords
This publication has 39 references indexed in Scilit:
- Systemic phencyclidine administration is associated with increased dopamine, GABA, and 5-HIAA levels in the dorsolateral striatum of conscious rats: an in vivo microdialysis studyJournal Of Neural Transmission-Parkinsons Disease and Dementia Section, 1994
- In vivo effects of local and systemic phencyclidine on the extracellular levels of catecholamines and transmitter amino acids in the dorsolateral striatum of anaesthetized ratsActa Physiologica Scandinavica, 1994
- Acute swim stress increases benzodiazepine receptors, but not GABAA or GABAB receptors, in the rat cerebral cortexNeurochemistry International, 1993
- Phencyclidine treatment in mice: Effects on phencyclidine binding sites and glutamate uptake in cerebral cortex preparationsJournal Of Neural Transmission-Parkinsons Disease and Dementia Section, 1993
- A steroid recognition site is functionally coupled to an expressed GABAA-benzodiazepine receptorEuropean Journal of Pharmacology: Molecular Pharmacology, 1990
- Effects of cortisol on GABAA receptor-mediated responses compared in guinea-pig ileum and rat cuneate nucleusNeuropharmacology, 1989
- Effects of the anticonvulsant taurine derivative, taltrimide, on membrane transport and binding of GABA and taurine in the mouse cerebrumNeuropharmacology, 1987
- Taurine and Gaba Binding in Mouse Brain Effects of Freezing, Washing and Triton X-100 Treatment on MembranesInternational Journal of Neuroscience, 1987
- Stress-induced increase in 3,4-dihydroxyphenylacetic acid (DOPAC) levels in the cerebral cortex and in n. accumbens: Reversal by diazepamLife Sciences, 1978