The Specificity and Cross-Reactivity of Antimyelin Antibodies as Determined by Sequential Adsorption Analysis

Open Access
Publisher Website
Google Scholar
Abstract
Through the process of sequential adsorption analysis of antimyelin I antibodies, raised in rabbits, rat myelin I was shown to possess a class of determinants distinct from synaptosomes and brain mitochondria, a class shared with synaptosomes but not brain mitochondria, and a class shared with synaptosomes and brain mitochondria as well as with liver mitochondria. Myelin and synaptosomes were thus distinguished immunologically from mitochondria and from each other. Although most of the antimyelin I antibodies also cross-reacted with myelin II some remained distinctive for myelin I, thus revealing a class of determinants in I not prominent in II. Crossreactions of the rabbit antirat-myelin antibodies with myelin I from different species in increasing order were bullfrog, 23%; chicken, 37%; rabbit, 50%; guinea pig, 50%; dog, 53%; human, 59%; neonatal rat, 61%; mouse, 72%; and hamster, 73%. The last three values were felt to be underestimated.

This publication has 0 references indexed in Scilit: