A catalog of human cDNA expression clones and its application to structural genomics
Open Access
- 17 August 2004
- journal article
- research article
- Published by Springer Nature in Genome Biology
- Vol. 5 (9) , R71
- https://doi.org/10.1186/gb-2004-5-9-r71
Abstract
We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.Keywords
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