SUSCEPTIBILITY TO MITOMYCIN C AND LECITHINASE ACTIVITIES OF CLOSTRIDIUM OEDEMATIENS (C. NOVYI) TYPES B AND D

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Abstract
Summary In tests with broth-culture products of Clostridium oedematiens, none of 15 type-B strains showed β-toxin lecithinase activities exceeding 20 egg-units per ml, whereas 12 of 13 type-D strains consistently produced much greater amounts of the lecithinase. The types also differed in their susceptibility to lysis by mitomycin C (MC). Of 13 type-D strains tested, 12 were sensitive to MC at a concentration of 1 or 2 μg per ml, whilst 14 of 15 type-B strains were insensitive. Phage-like particles were observed in the MC-lysates of some type-D strains. No type-specific differences in the production of indole or the fermentation of maltose were demonstrated.

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