Comparison of serum and whole blood levels of cytomegalovirus and Epstein–Barr virus DNA
- 21 September 2008
- journal article
- research article
- Published by Wiley in Transplant Infectious Disease
- Vol. 10 (5) , 308-315
- https://doi.org/10.1111/j.1399-3062.2008.00313.x
Abstract
The monitoring of viral DNA levels after transplantation is crucial for prevention of complications from cytomegalovirus (CMV) or Epstein–Barr virus (EBV) infection but there is no consensus as to which matrix is the most adequate. To compare serum and whole blood (WB) as specimens for measuring viral DNA, clinical samples from a 3‐year period were studied, with focus on cases where serum and WB were drawn on the same day. In 1896 paired serum and WB samples, CMV DNA was detected in both specimen types in 472 samples with 0.18 log higher levels (PP50% of WB samples and at 30 times higher levels than in serum, and that post‐transplantation lymphoproliferative disorder (PTLD) may develop without further increase of EBV DNA in WB. Identification of PTLD may require EBV DNA testing in both specimen types or complementary tests.Keywords
This publication has 33 references indexed in Scilit:
- Quantification of a low cellular immune response to aid in identification of pediatric liver transplant recipients at high‐risk for EBV infectionClinical Transplantation, 2006
- Epstein–Barr viremia levels after pediatric liver transplantation as measured by real‐time polymerase chain reactionPediatric Transplantation, 2005
- Cytomegalovirus infection after allogeneic stem cell transplant in childrenPediatric Transplantation, 2005
- Quantification of Epstein-Barr virus load in Argentinean transplant recipients using real-time PCRJournal of Clinical Virology, 2004
- Lymphoproliferative disease after allogeneic stem cell transplantation—pre-emptive diagnosis by quantification of Epstein–Barr virus DNA in serumJournal of Clinical Virology, 2003
- Measurement of Epstein-Barr Virus DNA Loads in Whole Blood and Plasma by TaqMan PCR and in Peripheral Blood Lymphocytes by Competitive PCRJournal of Clinical Microbiology, 2003
- Randomized controlled trial of oral ganciclovir versus oral acyclovir after induction with intravenous ganciclovir for long-term prophylaxis of cytomegalovirus disease in cytomegalovirus-seropositive liver transplant recipientsTransplantation, 2003
- SERIAL MEASUREMENT OF EPSTEIN-BARR VIRAL LOAD IN PERIPHERAL BLOOD IN PEDIATRIC LIVER TRANSPLANT RECIPIENTS DURING TREATMENT FOR POSTTRANSPLANT LYMPHOPROLIFERATIVE DISEASE1Transplantation, 1998
- Donated organ as a source of cytomegalovirus in orthotopic liver transplantationJournal of Medical Virology, 1992
- Cytomegalovirus infection and disease after liver transplantationDigestive Diseases and Sciences, 1992