Detection of Seedborne Plant Viruses Using Serologically Specific Electron Microscopy

Publisher Website
Google Scholar
Abstract
Serologically specific electron microscopy (SSEM) was used to detect and identify virus particles in crude extracts of seeds infected with tobacco ringspot virus (TRSV), barley stripe mosaic virus (BSMV), lettuce mosaic virus (LMV) and soybean mosaic virus (SMV) [from soybean, barley, lettuce and soybean, respectively]. The seeds were in halves and one half was assayed for virus using SSEM. The remaining seed halves were combined with healthy seed material in mixtures of 1:10, 1:100, 1:1000 and 1:10,000 (wt/wt) and also were assayed using SSEM. Virus particles were detected in half seeds in assays using specific antiserum. Particles of TRSV, BSMV and SMV were detected in 1 part of infected seed extract per 1000 parts of healthy seed extract; LMV was detected in one part of infected seed extract per 100 parts of healthy seed extract.