ORIGIN OF THE RAT MESANGIAL PHAGOCYTE AND ITS EXPRESSION OF THE LEUKOCYTE COMMON ANTIGEN

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 51  (5) , 515-523
Abstract
Previously reported was a subpopulation of cells in the rat glomerular mesangium that are phagocytic, display Ia antigens, and stimulate lymphocytes. Total-body irradiation apparently induces a progressive loss of the Ia+ mesangial cells, which is complete by 3 days. Experiments utilizing kidney shielding and in vitro exposure to irradiation have established that the effect is not mediated via radiation sensitivity of the mesangial phagocyte. Transplantation of syngeneic bone marrow cells results in a restoration of Ia+ mesangial cells in 3 wk, establishing their extrarenal origin. Employing monoclonal antibodies to rat leukocyte common (LC) antigen, a larger population of mesangial phagocytes were quantified: the Ia+ cells are a subset of the glomerular LC+ cells. In tissue culture, anti-LC antibody labels 100% of the phagocytic cells obtained from perfused glomeruli. Ultrastructural analysis, utilizing immunoperoxidase techniques, has localized the LC+ cell to the glomerular mesangium.

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