A simplified procedure for evaluation and storage of isoelectric focusing gels prior to second‐dimension electrophoresis
- 1 January 1985
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 6 (6) , 296-298
- https://doi.org/10.1002/elps.1150060610
Abstract
A simple method of stain fixation of polyacrylamide isoelectric focusing tube gels is described. Gel handling and storage are simplified by fixation and second‐dimension quality is markedly improved over that from the original procedure involving quick freezing and frozen storage of the gel.Keywords
This publication has 7 references indexed in Scilit:
- Gel gradient electrophoresis, isoelectric focusing and two-dimensional techniques in horizontal, ultrathin polycrylamide layersJournal of Biochemical and Biophysical Methods, 1980
- Visualization of proteins after isoelectric focusing during two-dimensional gel electrophoresisAnalytical Biochemistry, 1979
- Composition of an isoelectric focusing gel yielding a broad pH gradientAnalytical Biochemistry, 1978
- High resolution two-dimensional electrophoresis of basic as well as acidic proteinsCell, 1977
- Some improvements in two-dimensional gel electrophoresis of proteinsAnalytical Biochemistry, 1977
- Two-dimensional gel electrophoresis of membrane proteinsBiochemistry, 1976