Abstract
Starch gel electrophoresis of leaves of diploid and more particularly tetraploid orchard grass (Dactylis glomerata L.) from cultivars as well as natural populations disclosed several anodal stable bands with fast migration and phosphatasic activity. Six different phenotypes with one band, many others with three regular bands or six bands (only in the tetraploid individuals), were observed, showing the dimeric structure of the enzymes. Inheritance studies showed one polymorphic locus with six codominant alleles AcPH 11.00, AcPH 10.95, AcPH 10.90, AcPH 10.88, AcPH 11.02and AcPH 11.04with differences in enzymatic expression. AcPH1.00and AcPH 10.90have been found in the two ploidy levels. Results from the tetraploid progenies involved tetravalents in meiosis for individuals originating from cultivars but bivalents in the wild plants. The two loci AcPH 1 and GOT 1 (this last one coding for a glutamate oxaloacetate transaminase) are not linked and seem to be located on different chromosomes.