Growth and Alkaloid Production in Suspension Cultures of Hyoscyamus muticus as Influenced by Various Cultural Parameters

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Abstract
Suspension cultures of H. muticus L. were raised in RT medium supplemented with NAA [naphthalene acetic acid] 10-5 M. Effects of pH, vitamins, sucrose, sodium and potassium succinate, chemical precursors and phytohormones on growth, morphogenesis and total tropane alkaloid of pharmaceutical interest production were examined. Of these, low pH, sodium succinate, precursors and IAA enhanced alkaloid production. Alkaloid synthesis was higher during stationary phase and was independent of morphogenesis.